• April 16th, 2016

Bacterial techniques, plasmid isolation, digestion and gel electrophoresis

Paper, Order, or Assignment Requirements

Introduction

“Introduce” all the techniques used – handling bacteria (why is this important, what is it used for), plasmid DNA (what is it used for, mention antibiotic resistance) – it is the first step in cloning and DNA manipulation, restriction (what are restriction enzymes, why are they used), gel electrophoresis (what’s the principle behind the process and applications).

Aims of the experiments (to learn basic techniques of manipulating bacteria, isolate plasmid DNA, cut it (digest) and verify the results through gel electrophoresis).

Methods

Describe the procedures performed in your own words.

Results and Discussion

“Discuss” the procedures and your results (use any illustrations you think might be helpful from the PDF protocols of the course and add your gel picture to comment on). Use the “tasks” section of the protocols as a guideline of what to mention, comment and interpret your results.

Conclusion

Where your experiments successful and did you achieve your aim (mentioned in the introduction.
References

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